鸡包涵体肝炎病原检测及病理学观察

doi:10.11843/j.issn.0366-6964.2015.02.014

Abstract

Abstract:

One strain of the inclusion body hepatitis virus (IBHV) was isolated and identified based on pathological changes，PCR and sequence analysis in sick chicken.The DNA of the IBHV was extracted from allantoic fluid obtained from SPF egg，which was inoculated with the sick chicken liver.The primers were designed based on the conserved hexon sequence of avian adenovirus.The PCR product of avian adenovirus was amplified and sequenced.The fatty degeneration，karyopyknosis，cell necrosis and basophilic inclusion bodies were observed in liver tissue from sick chicken.The sequencing results indicated that the virus shared 98.6% identity with FAV-4 in group Ⅰ and 68.7%-96.9% identity with other serotypes in group Ⅰ，and 44.6% identity with hemorrhagic enteritis virus in group Ⅱ and egg drop syndrom virus in group Ⅲ.These results confirmed that the isolated virus is a member of fowl adenovirus groups，and can cause chicken inclusion body hepatitis.Our research provides the basis for isolation and identification of group Ⅰ avian adenovirus.

CLC Number:

S852.659.1

WANG Wan，WANG Yan-hong，LIU Yi，JIN Wen-jie，SHI Huo-ying. Pathogenic Detection and Pathological Observation of Inclusion Body Hepatitis in Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 273-278.

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Pathogenic Detection and Pathological Observation of Inclusion Body Hepatitis in Chicken

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